• Species Reactivity

  Mouse

• Specificity

  Detects mouse Marapsin in ELISAs.  In sandwich immunoassays, no cross-reactivity or interference with recombinant human Marapsin or recombinant mouse Prostasin was observed.

• Source

  Monoclonal Rat IgG    _2B Clone # 246406

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse Marapsin/Pancreasin    
  Ala23-Thr290    
  Accession # Q8BJR6

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Mouse Marapsin/Pancreasin Sandwich Immunoassay

  Reagent    

• ELISA Capture (Matched Antibody Pair)

  2-8 μg/mL 

  Mouse Marapsin/Pancreasin Antibody (Catalog # )

• ELISA Detection (Matched Antibody Pair)

  0.1-0.4 μg/mL 

  Mouse Marapsin/Pancreasin Biotinylated Antibody (Catalog # )

• ELISA Standard

   

  Recombinant Mouse Marapsin/Pancreasin Protein, CF (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Marapsin/Pancreasin

Marapsin, Pancreasin, and channel-activating protease 2 (CAP-2), encoded by the Prss27 gene, are different names given for the same serine protease that is expressed strongly in the pancreas (1). The mouse protein is synthesized with a signal peptide (amino acid residues 1 - 22), a pro peptide (residues 23 - 37) and a mature chain (residues 38 - 290) corresponding to the serine protease domain. The full-length protein was expressed and the secreted protein purified. The N-terminal sequencing results indicate that the purified protein is a disulfide bond-linked dimer formed between the pro peptide and the mature chain. The active enzyme has low activity against peptide substrates tested, but high activity against thioester substrates. The peptidase activity is inhibited by 20 mM benzamidine.

• References:

1. Bhagwandin, V.J. et al. (2003) J. Biol. Chem. 278:3363.

• Entrez Gene IDs:

  83886 (Human); 213171 (Mouse)

• Alternate Names:

  CAP-2; CAPH2; channel-activating protease 2; EC 3.4.21; EC 3.4.21.-; EC 3.4.21.4; EC 3.4.21.43; Marapsin; MPN; MPNMarapsin; Pancreasin; protease, serine 27; PRSS27; serine protease 27