• Species Reactivity

  Human

• Specificity

  Detects human NrCAM in direct ELISAs and Western blots.

• Source

  Monoclonal Mouse IgG    _2A Clone # 297909

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human NrCAM isoform 3    
  Leu30-Asn600 (Ala526Pro)    
  Accession # Q14CA1

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 μg/mL

  Recombinant Human NrCAM Fc Chimera (Catalog # )    
  under non-reducing conditions only

• 
  

• Human NrCAM Sandwich Immunoassay

  Reagent    

• ELISA Capture (Matched Antibody Pair)

  2-8 μg/mL 

  Human NrCAM Antibody (Catalog # )

• ELISA Detection (Matched Antibody Pair)

  0.1-0.4 μg/mL 

  Human NrCAM Isoform 3 Biotinylated Antibody (Catalog # )

• ELISA Standard

   

  Recombinant Human NrCAM Fc Chimera Protein, CF (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NrCAM

NgCAM related cell adhesion molecule (NrCAM), also known as Bravo, belongs to the L1 family of cell adhesion molecules, which also include L1, neurofascin and close homolog of L1 (CHL-1) (1). These molecules are type I transmembrane proteins that have 6 Ig-like domains and 4 - 5 fibronectin type III-like domains in their extracellular domain. They also shared a conserved cytoplasmic region containing an ankyrin-binding site. L1 family cell adhesion molecules are expressed primarily in the nervous system where they share overlapping functions in controlling axonal growth and guidance (1, 2). NrCam mediates homophilic adhesion as well as heterophilic adhesion with a number of neuronal adhesion molecules including contactin, TAG-1/contactin-2, neurofascin and receptor tyrosine phosphatase beta (RPTP beta ) (3-5). NrCAM has been implicated in the axogenesis of multiple neuronal populations including sensory axons and commissural axons in the spinal cord of chick embryos. It also plays a role in cerebellar granule cell development, and is required in node of Ranvier formation (5, 6).

• References:

1. Hortsch, M. (2000) Mol. Cell. Neurosci. 15:1.

2. Stoeckli, E.T. and L.T. Landmesser (1995) Neruon 14:1165.

3. Mauro, V.P. et al. (1992) 119:191.

4. Fustig, M. Sakurai, T. and M. Grumet (1999) Dev. Bio. 209:340.

5. Sakurai, T. et al. (2001) J. Cell Bio. 154:1259.

6. Custer, A.W. et al. (2003) J. Neuroscience 23:10032.

• Long Name:

  Neuronal Cell Adhesion Molecule

• Entrez Gene IDs:

  4897 (Human); 319504 (Mouse)

• Alternate Names:

  Bravo; hBravo; KIAA0343Neuronal surface protein Bravo; MGC138845; MGC138846; neuronal cell adhesion molecule; NgCAM-related cell adhesion molecule; ng-CAM-related; NrCAM; nr-CAM