• Species Reactivity

  Human

• Specificity

  Detects human PI 3?Kinase C2 beta in direct ELISAs and Western blots.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  E. coli-derived recombinant human PI 3?Kinase C2 beta    
  Ser2-Ser134    
  Accession # O00750

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  2 μg/mL

  See below

• 
  

• Immunocytochemistry

  5-15 μg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.2 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PI 3-Kinase C2 beta

PIK3C2 beta (Phosphadidylinositol-4-phosphate 3 kinase C2 domain-containing beta subunit; also HsC2-PI3K) is a 175-185 kDa member of the Class II PI3/PI4 kinase family of enzymes. It is widely expressed, being found in neurons, urinary transitional and columnar epithelium, and fibroblasts. PIK3C2 beta likely participates in growth factor, integrin and chemokine signaling by interacting with select cell membrane receptors, and appears to phosphorylate both phosphatidylinositol (PI) and PI 4?monophosphate upon activation. Human PIK3C2 beta is 1634 amino acids (aa) in length. It contains an N-terminal Pro-rich region (aa 156-174), two PI3K Class II domains (aa 622-794 and 1505-1627), a PI3K catalytic region (aa 987-1340), one PX domain (aa 1365-1481) and an NLS (aa 1555-1565). There are two potential C2 beta isoform variants. One shows a deletion of aa 850-877, while another contains a three aa insertion after Gln1016. Over aa 2-134, human PIK3C2 beta ?shares 91% aa identity with mouse PIK3C2 beta.

• Long Name:

  Phosphatidylinositol-4-Phosphate 3-Kinase C2 Domain-containing Subunit beta

• Entrez Gene IDs:

  5287 (Human); 240752 (Mouse); 289021 (Rat)

• Alternate Names:

  C2-PI3K; C2-PI3KDKFZp686G16234; EC 2.7.1; EC 2.7.1.154; phosphatidylinositol 3-kinase C2 domain-containing beta polypeptide; phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing subunit beta; Phosphoinositide 3-kinase-C2-beta; phosphoinositide-3-kinase, class 2, beta polypeptide; PI 3Kinase C2 beta; PI 3-Kinase C2 beta; PI3K-C2beta; PI3K-C2-beta; PIK3C2B; PTDINS-3-kinase C2 beta; PtdIns-3-kinase C2 subunit beta