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Product Brief

• Overview

  Product Name	10 nm Colloidal Gold Labeled Protein A
  SKU/Catalog Number	GA1054
  Description	Protein A is a 42 kDa surface protein originally found in the cell wall of the bacteria Staphylococcus aureus. It is encoded by the spa gene and its regulation is controlled by DNA topology, cellular osmolarity, and a two-component system called ArlS-ArlR. It has found use in biochemical research because of its ability to bind immunoglobulins. It is composed of five homologous Ig-binding domains that fold into a three-helix bundle. Each domain is able to bind proteins from many mammalian species, most notably IgGs (Table 1). It binds the heavy chain within the Fc region of most immunoglobulins and also within the Fab region in the case of the human VH3 family. Through these interactions in serum, where IgG molecules are bound in the wrong orientation (in relation to normal antibody function), the bacteria disrupts opsonization and phagocytosis. Recombinant Protein A is produced in E.coli and essentially functions the same as native Protein A. Protein A may be conjugated with various reporter molecules, including fluorescent dyes (FITC), enzyme markers (peroxidase, b-galactosidase, and alkaline phosphatase), biotin, and colloidal gold without affecting the antibody binding site on the molecule. These conjugates are used to detect immunoglobulins in various immunochemical assays including Western blotting, immunohistochemistry, and ELISA applications.br> Protein A is conjugated to 10 nm colloidal gold. This gold conjugated protein A generates electron dense particles that are visible using electron microscopy.
  Cite This Product	10 nm Colloidal Gold Labeled Protein A (Boster Biological Technology, Wuhan, CHINA, Catalog # GA1054)
  Application	EM, IHC, ICC
  Conjugate	10 nm Colloidal Gold
  Pack Size	0.5ml/1ml

  Properties

  Storage	At 4°C for one year.

Instructions

膠體金稀釋液：PBS 或 TBS 緩沖液，加入試劑級 BSA 至 1%。 使用上述膠體金稀釋液，免疫電鏡按 1：20—50 稀釋，免疫組化按 1：50—100 稀釋。膠體金 稀釋后溶液應(yīng)于當(dāng)天使用，未用完的應(yīng)棄置。