•  Enzyme Commission (EC) Number  1.1.99.11  ( BRENDA | IUBMB )

•  MDL number  MFCD00130905

• Popular Documents:  Specification Sheet (PDF)

產品名稱

包裝

250 units in glass bottle

Unit Definition

One unit will convert 1.0 μmole-fructose to 5-ketofructose per min at pH 4.5 at 37 °C.

Physical form

supplied as a lyophilized powder containing approx 80% stabilizers, sugars, amino acids and BSA

Application

This enzyme is also used in a number of basic research projects to examine the electrochemical properties of enzyme-catalyzed electrode reactions called bioelectrocatalysis.This enzyme is useful for enzymatic determination of D-fructose in clinical analysis.

Biochem/physiol Actions

D-fructose dehydrogenase is a heterotrimeric membrane-bound enzyme commonly seen in various Gluconobacter sp. especially in Gluconobacter japonicus (Gluconobacter industrius). It has a molecular mass of ca. 140 kDa, consisting of subunits I (67kDa), II (51 kDa), and III (20 kDa) and catalyzes the oxidation of D-fructose to produce 5-keto-D-fructose. The enzyme is a flavoprotein-cytochrome c complex with subunits I and II covalently bound to flavin adenine dinucleotide (FAD) and heme C as prosthetic groups, respectively.

Physical properties

Isoelectric point : 5.0 ± 0.1
Michaelis constant : 5 x 10￣³M (D-Fructose)
Inhibitors : Ag⁺, Hg⁺⁺, SDS
Optimum pH : 4.0
Optimum temperature : 37°C
pH Stability : pH 4.0 – 6.0 (25°C, 16hr)
Thermal stability : Below 40°C (pH 4.5, 15min)

性質