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| Description | |
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| Overview | The ProteoExtract? Phosphopeptide TiO2 Enrichment Kit is a useful tool for enrichment of phosphorylated peptides from complex samples by a highly selective Titanium oxide solid phase. It enhances the ability to routinely identify and characterize large numbers of phosphorylated species within complex protein mixtures taking advantage of a novel titanium dioxide material. Captured and eluted phosphopeptide fractions are ready for MALDI- and ESI- mass spectrometry analysis. |
| Catalogue Number | 539722 |
| Brand Family | Calbiochem? |
| Application Data |  Titanium dioxide allows for selective and sensitive phosphopeptide enrichment with low background from complex mixtures. A complex peptide mixture derived from a tryptic digest of porcine liver extract was spiked with α-casein and two synthetic phosphopeptides (see below for concentrations). The samples were subsequently processed using the ProteoExtract? Phosphopeptide Enrichment TiO2 Kit as outlined in the Detailed Protocol. Mass spectrometry analysis was performed using ESI-LC/MS equipment operated in positive mode. (A) Unprocessed sample (B) Phosphopeptides recovered using the ProteoExtract? Phosphopeptide Enrichment TiO2 Kit. Spiked Phosphopeptides: 1. 50 nM Angiotensin ~6 pmol/125 μl; ~7 ng/125 μl 2. 67 nM Calcineurin Substrate ~8 pmol/125 μl; ~18 ng/125 μl 3. 140 nM α-Casein ~18 pmol/125 μl; ~400 ng/125 μl |
| Materials Required but Not Delivered | ? Micropipettes and tips; 10 μl, 200 μl, and 1 ml ? Microcentrifuge and rotor for >10,000 x g ? 1.5 ml microcentrifuge tubes |
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| Detection method | Mass Spectrometry |
| Form | 100 Isolations |
| Format | Phosphopeptide enrichment |
| Kit contains | TiO? Phosphobind Resin, 10X TiO? Phosphobind Buffer, Wash Buffer 1, Wash Buffer 2, TiO? Elution Buffer, Dihydroxybenzoic Acid, and a user protocol. |
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| Intended use | The ProteoExtract? Phosphopeptide Enrichment TiO? Kit is designed to enrich phosphorylated peptides from complex samples using a solid phase, highly selective, titanium oxide. The enriched phosphopeptide fraction is directly compatible with downstream analysis by MALDI and ESI mass spectrometry. |
| Storage and Shipping Information | |
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| Ship Code | Blue Ice Only |
| Toxicity | Multiple Toxicity Values, refer to MSDS |
| Hazardous Materials Attention: | Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges. |
| Storage | +2°C to +8°C |
| Storage Conditions | Upon arrival store the entire contents of the kit at 4°C. Following reconstitution of the Dihydroxybenzoic Acid refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage; reconstituted Dihydroxybenzoid Acid is stable for up to 6 weeks at 4°C or for up to 6 months at -20°C. |
| Do not freeze | Ok to freeze |
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| Kit contains | TiO? Phosphobind Resin, 10X TiO? Phosphobind Buffer, Wash Buffer 1, Wash Buffer 2, TiO? Elution Buffer, Dihydroxybenzoic Acid, and a user protocol. |
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