詳細(xì)說明
Replacement Information |
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重要規(guī)格表
Detection Methods |
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Colorimetric |
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Description | |
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Overview | A sensitive spectrophotometric assay (340 nm) for the measurement of glutathione peroxidase (GPx) activity in plasma, erythrocyte lysates, tissue homogenates, and cell lysates. Assay range: 50-344 nmol/min/ml. Contains sufficient reagents for 96 tests. |
Catalogue Number | 353919 |
Brand Family | Calbiochem? |
Materials Required but Not Delivered | ? A plate reader with a 340 nm filter ? Adjustable pipettors and a repeat pipettor ? A source of pure water. Glass distilled water or HPLC-grade water is acceptable ? PBS ? Homogenization buffer (cell and tissue preparation) |
References | |
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References | Ursini, F., et al. 1985. Biochim. Biophys. Acta 839, 62. Forstrom, J.W., et al. 1978. Biochem. 17, 2639. Paglia, D.E., et al. 1967. J. Lab. Clin. Med. 70, 158. |
Product Information | |
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Detection method | Colorimetric |
Form | 96 Tests |
Format | 96-well plate |
Kit contains | Assay Buffer, Sample Buffer, Glutathione Peroxidase (Control), Co-Substrate Mixture, Cumene Hydroperoxide, 96 Well Plate, Plate Cover, and a user protocol. |
Positive control | Bovine erythrocyte glutathione peroxidase |
Applications |
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Biological Information | |
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Assay range | 50-344 nmol/min/ml |
Assay time | 2 h |
Sample Type | Cells, tissues, plasma |
Physicochemical Information |
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Dimensions |
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Materials Information |
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Toxicological Information |
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Safety Information according to GHS |
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Safety Information |
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Product Usage Statements |
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Storage and Shipping Information | |
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Ship Code | Blue Ice Only |
Toxicity | Standard Handling |
Storage | -20°C |
Storage Conditions | Upon arrival store the entire contents of the kit at -20°C. |
Do not freeze | Ok to freeze |
Packaging Information |
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Transport Information |
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Supplemental Information | |
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Kit contains | Assay Buffer, Sample Buffer, Glutathione Peroxidase (Control), Co-Substrate Mixture, Cumene Hydroperoxide, 96 Well Plate, Plate Cover, and a user protocol. |
Specifications |
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