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Glutathione Peroxidase Assay Kit

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    Detection Methods
    Colorimetric

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    Description
    OverviewA sensitive spectrophotometric assay (340 nm) for the measurement of glutathione peroxidase (GPx) activity in plasma, erythrocyte lysates, tissue homogenates, and cell lysates. Assay range: 50-344 nmol/min/ml. Contains sufficient reagents for 96 tests.
    Catalogue Number353919
    Brand FamilyCalbiochem?
    Materials Required but Not Delivered? A plate reader with a 340 nm filter
    ? Adjustable pipettors and a repeat pipettor
    ? A source of pure water. Glass distilled water or HPLC-grade water is acceptable
    ? PBS
    ? Homogenization buffer (cell and tissue preparation)
    References
    ReferencesUrsini, F., et al. 1985. Biochim. Biophys. Acta 839, 62.
    Forstrom, J.W., et al. 1978. Biochem. 17, 2639.
    Paglia, D.E., et al. 1967. J. Lab. Clin. Med. 70, 158.
    Product Information
    Detection methodColorimetric
    Form96 Tests
    Format96-well plate
    Kit containsAssay Buffer, Sample Buffer, Glutathione Peroxidase (Control), Co-Substrate Mixture, Cumene Hydroperoxide, 96 Well Plate, Plate Cover, and a user protocol.
    Positive controlBovine erythrocyte glutathione peroxidase
    Applications
    Biological Information
    Assay range50-344 nmol/min/ml
    Assay time2 h
    Sample TypeCells, tissues, plasma
    Physicochemical Information
    Dimensions
    Materials Information
    Toxicological Information
    Safety Information according to GHS
    Safety Information
    Product Usage Statements
    Storage and Shipping Information
    Ship CodeBlue Ice Only
    ToxicityStandard Handling
    Storage-20°C
    Storage ConditionsUpon arrival store the entire contents of the kit at -20°C.
    Do not freezeOk to freeze
    Packaging Information
    Transport Information
    Supplemental Information
    Kit containsAssay Buffer, Sample Buffer, Glutathione Peroxidase (Control), Co-Substrate Mixture, Cumene Hydroperoxide, 96 Well Plate, Plate Cover, and a user protocol.
    Specifications
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